Marwa Eltoweissy
Georg August Medical University of Göttingen, Germany and Alexandria University, Egypt
Title: Short-time increase of glucose concentration in PDS results in extensive removal and high glycation level
Biography
Biography: Marwa Eltoweissy
Abstract
Background: Renal diseases constitute a major health risk in all societies. The prevalence of end-stage renal disease (ESRD) in adult European populations is above 10% with tendency to increase, posing a serious health threat. The treatment of ESRD involved the use of various dialysis procedures or kidney transplantation. Continuous ambulatory peritoneal dialysis (CAPD) uses the well flow peritoneum as a biological semipermeable membrane without an extra corporal blood circulation. Glucose solution is commonly used as dialysate compound. In our present study, we investigated the impact of short-time alteration of the glucose concentration and the osmolarity of the peritoneal dialysis solution (PDS) on protein removal.
Methods: Peritoneal dialysis liquids (PDL) were collected from 19 well-characterized CAPD patients treated with two types of PDS. The patients were subjected to short-time changes (4 h) of glucose concentration of PDS. The depletion of the six-interfering high abundant proteins from the PDL samples was performed with the Multiple Affinity Removal LC Column-Human 6. The resulting protein fractions were analyzed by 2D gel electrophoresis, differential in gel electrophoresis, mass spectrometry and 2D western blot.
Results: Proteomics investigation of the PDL fractions after depletion allowed the identification of 198 polypeptides, which equate to 48 non-redundant proteins. Comparative analyses of 2D gel electrophoresis protein pattern revealed a clear correlation between protein removal, PDS glucose concentration and osmolarity. An increase for 4 h in the PDS osmolarity (with 43-51 mOsmol/L) resulted qualitatively in 18-23% more protein removal in PDL. Moreover, 2D western blot analyses of the protein glycation pattern showed that the short-time increase in PDS glucose concentration (45-50 mM) resulted in significant alteration of the advanced glycosylation end products (AGEs) pattern.
Conclusions: The data presented in this study shed light on the quality of the protein lose during CAPD due to the glucose concentration in used dialysate. Moreover, we could demonstrate that higher glucose concentration in dialysis solution results in increased AGEs.