Bioinformatics

Biography

Biography: Abdullah Essa Alsubhi

Abstract

Urinary tract infection (UTI) is one of the most prevalent bacterial infections in the world. It affects urinary tract system including bladder and kidney. Gram-negative bacteria is a major cause, particularly Escherichia coli (E. coli), which is considered a main causative agent for 80-90% of community-acquired infection and for about 40% of nosocomial UTI. Moreover, it is responsible for 25% of recurrent infection. The study of the genome identified and sequenced three E. coli strains causing UTIs and all strains showed higher expression of certain proteins; such as adhesion protein p, and toxin such as hemolysin and high expression of proteins responsible for iron acquisition. Proteomics is used to analyze and identify complete components of proteins and it can be used to distinguish between bacteria based on synthesized protein. In addition, proteomic is applicable to identify possible target of therapy. This study aims to compare protein profiles of E. coli from different UTI patients and identify possible unique proteins signature for future biomarker studies. Seven urine samples were taken from different UTI patients and the pathogenic agent among those patients was E. coli. Urine E. coli isolates were analyzed by 1D SDS-PAGE, 2DGE and LC-MS/MS and three interesting spots were selected for protein identification by LC-MS/MS. Many significant differences were observed in protein profiles of E. coli isolates in both 1D SDS-PAGE and 2DGE regardless of type of E. coli species. Finally, it was concluded that E. coli isolates obtained from the different UTI patients had different protein profiles.