Bioinformatics

Biography

Biography: Mohammed Sulaiman Al-Hajou

Abstract

Urinary tract infection (UTI) is considered to be one of the most prevalent bacterial infections in the world predominantly affecting the bladder and the kidney; according to research, one in three women is affected by UTIs. Gram-negative bacteria are a major cause of such infections, particularly Escherichia coli (E. coli). E. coli was the main causative agent of 80-90% of community-acquired infection, about 40% of nosocomial UTI, and is responsible for 25% of recurrent infections. The field of proteomics has emerged as a great tool to analyze expressed proteins and to identify possible biomarkers associated with a number of pathological states, and to the same extent associated with bacterial pathogenesis. Researchers elsewhere are investigating E. coli proteomics profiles to identify possible biomarkers, however, protein profiles could vary environmental stress created by subculturing. Here we propose to answer the research questions, are there differences in protein profiles of E. coli originated from a sequential passage? To conduct this research, urine samples will be collected from individuals with recurrent UTI, sequentially subcultured, and analyzed using two-dimensional gel electrophoresis and mass spectrometry to identify any significant change in the protein profile of the bacteria. We hope to elucidate to the effect of passage on the protein profile of this common pathogen.