Bin Huang
Kaohsiung Medical University, Taiwan
Title: Application of embryogenic mouse as a platform in identifying human missing proteins
Biography
Biography: Bin Huang
Abstract
Missing proteins are those genes with detectable transcriptional mRNA while missed in detecting translational proteins either by mass spectrometry or Western blot. To date, missing proteins are thought as a “KEY” in triggering many early physiological responses and then disappear after these signaling cascades are turned on. That’s why missing proteins are always “MISSED” in numerous studies. Embryo contains many definitive genes to temporarily control subsequent organogenesis. Since the decoding of human and mouse whole genome, their proteins share high similarity in amino acid sequence. The embryos taken from pregnant mice for 6.5, 7, 7.5 and 8 days, the time for the onset of neuron plate formation, were analyzed by mass spectrometry. There were 17 missing proteins identified. The Western blot showed that NKX1 (Chr#4), PTCHD1 (Chr#X), OTOGL (Chr#12) and ASXL3 (Chr#20) were increased in day 7 and 7.5, while decreased in day 8. This transient expression indicates an association of missing proteins in neuron development. As a result, the embryonic mice could be the most suitable platform for detecting missing proteins and elucidating their role in organogenesis.